Zernike Phase Plate Configuration at Intermediate Lens Position on JEM2200FS
نویسندگان
چکیده
منابع مشابه
Transmission electron microscopy with Zernike phase plate.
The possibility of implementing a Zernike phase plate in a transmission electron microscope is investigated both theoretically and experimentally. The phase-retarding plate in the form of thin film with a hole in the center is positioned in the back-focal plane of the objective lens. The experiments show that the phase plate functions as predicted, producing a cosine-type phase contrast transfe...
متن کاملHigh Throughput Fabrication Process of a Zernike Phase Plate
In transmission electron microscopy (TEM) for biological and polymer samples, it is difficult to image with high contrast, since they are mostly composed of light elements and have similar density. One solution to enhance the contrast is utilization of phase contrast microscopy, which is realized in optical microscopy. Accordingly, many types of phase plates for electron microscopy have been pr...
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In cryo-electron microscopy (cryo-EM), vitreously frozen biological specimens can tolerate only a limited electron dose for imaging. Therefore, cryo-EM micrographs display weak contrast and a poor signal-to-noise ratio. This limits data interpretability and prevents efficient application of cryo-EM to a wider range of biological problems. Continued efforts have been invested in imaging methods ...
متن کاملZernike phase plate cryoelectron microscopy facilitates single particle analysis of unstained asymmetric protein complexes.
Single particle reconstruction from cryoelectron microscopy images, though emerging as a powerful means in structural biology, is faced with challenges as applied to asymmetric proteins smaller than megadaltons due to low contrast. Zernike phase plate can improve the contrast by restoring the microscope contrast transfer function. Here, by exploiting simulated Zernike and conventional defocused...
متن کاملZernike phase-contrast x-ray microscope with pseudo-Kohler illumination generated by sectored (polygon) condenser plate
Zernike phase contrast x-ray microscope has been developed at the undulator beamline 20XU and 47XU of SPring-8. The system consists of a pseudo-Köhler-illuminating system, a Fresnel zone plate objective with outermost zone width of 100 nm, a Zernike phase plate (0.96-m-thick tantalum, /4 or 3/4 phase-shifter at 8 keV) installed at the back-focal plane of the objective, and a visible-light co...
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ژورنال
عنوان ژورنال: Microscopy and Microanalysis
سال: 2015
ISSN: 1431-9276,1435-8115
DOI: 10.1017/s1431927615011496